Worcester centre Ben Te'o has been ruled out of England's three-Test South Africa tour.
England head coach Eddie Jones has made three changes to his original 34-man squad, with Te'o sidelined due to what the Rugby Football Union described as a "small repair to quad muscle".
Sale Sharks back Cameron Redpath and flanker Jack Willis -- who will be out of action for at least nine months -- are also out.
The RFU announced call-ups for uncapped Gloucester full-back Jason Woodward, Northampton back Piers Francis and Wasps number eight Nathan Hughes.
England squad for South Africa Tour: Mike Brown (Harlequins) Danny Cipriani (Wasps) Luke Cowan-Dickie (Exeter Chiefs) Tom Curry (Sale Sharks) Elliot Daly (Wasps) Ben Earl (Saracens) * Nathan Earle (Saracens) * Owen Farrell (Saracens) George Ford (Leicester Tigers) Piers Francis (Northampton Saints) Ellis Genge (Leicester Tigers) Jamie George (Saracens) Jonny Hill (Exeter Chiefs) * Nathan Hughes (Wasps) Nick Isiekwe (Saracens) Maro Itoje (Saracens) Joe Launchbury (Wasps) Alex Lozowski (Saracens) Joe Marler (Harlequins) Jonny May (Leicester Tigers) Chris Robshaw (Harlequins) Dan Robson (Wasps) * Brad Shields (Hurricanes/ Wasps) * Sam Simmonds (Exeter Chiefs) Kyle Sinckler (Harlequins) Henry Slade (Exeter Chiefs) Denny Solomona (Sale Sharks) Ben Spencer (Saracens) * Billy Vunipola (Saracens) Mako Vunipola (Saracens) Harry Williams (Exeter Chiefs) Mark Wilson (Newcastle) Jason Woodward (Gloucester Rugby) * Ben Youngs (Leicester Tigers).
(* denotes uncapped)
Unavailable for selection: Danny Care (Harlequins) Jack Clifford (Harlequins) Dan Cole (Leicester Tigers) Tom Dunn (Bath Rugby) Charlie Ewels (Bath Rugby) Dylan Hartley (Northampton Saints) James Haskell (Wasps) Jonathan Joseph (Bath Rugby) George Kruis (Saracens) Courtney Lawes (Northampton Saints) Alex Lewington (London Irish) Harry Mallinder (Northampton Saints) Joe Marchant (Harlequins) Jack Nowell (Exeter Chiefs) Beno Obano (Bath Rugby) Cameron Redpath (Sale Sharks) Semesa Rokoduguni (Bath Rugby) Will Spencer (Worcester Warriors) Ben Te'o (Worcester Warriors) Manu Tuilagi (Leicester Tigers) Sam Underhill (Bath Rugby) Anthony Watson (Bath Rugby) Richard Wigglesworth (Saracens) Jack Willis (Wasps).