The charity basketball exhibition will take place at the Sprint Center in Kansas City, Missouri, on Oct. 22, Kansas coach Bill Self confirmed.
"I think it's a great opportunity for two schools to do something for the betterment of others," Self said. "This does not have one thing to do with playing Missouri."
Self said they're hoping to raise more than $1 million for hurricane relief. The proceeds would go toward relief for hurricane victims.
NCAA rules allow Division I teams to play a limited number of closed scrimmages against one another, but not exhibitions.
Missouri and Kansas ended their heated Border War rivalry in 2012 when the Tigers left the Big 12 for the SEC.
ESPN's Mitch Sherman contributed to this report.